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20 Urinary System Histology

Structure of the Nephron

The nephron is the functional unit of the kidney.  Each kidney contains roughly one million nephrons.   The illustration on the left in the figure below depicts the kidney as it would look if sectioned along its long axis and shows how the nephrons are arranged between the two major regions of the kidney, the cortex and the medulla.

a sectioned kidney (left) and two nephrons (right) with shading indicating the cortex and the medulla.
On the left is an illustration of a kidney sectioned along its long axis. Two nephrons are depicted at the top. The illustration on the right shows an enlarged view of two nephrons, with the cortex shaded tan and the medulla shaded pink. The nephron on the left is a cortical nephron; the nephron on the right is a juxtamedullary nephron. Left figure adapted from figure 19.1c; right figure adapted from figure 19.1e in Human Anatomy: An Integrated Approach by Dee Silverthorn, 8th edition.

The figure on the right shows an enlarged view of two nephrons.  We will focus on the structure and function of juxtamedullary nephrons (shown on the right).  Juxtamedullary nephrons have long loops of Henle that are located in the medulla, and they play a key role in the concentration of urine.

The next figure illustrates blood vessels and nephrons and identifies the different part of the nephron.  As the figure shows, some parts of the nephron are located in the cortex, and some are found in the medulla.

tracing of nephrons (blue) and blood vessels(red) in the kidney cortex and medulla
An illustration tracing the outline of blood vessels (red) and nephrons (blue) in the kidney. The different parts of the nephron are indicated, as is the boundary between the cortex and the medulla.

The first part of the nephron is the renal corpuscle, which consists of a tuft of the capillaries, the glomerulus (red), surrounded by Bowman’s capsule (blue).  The rest of the nephron is called the renal tubule.  The entire renal tubule is formed by a simple epithelium, and epithelial transport is important in the function of the renal tubule.  The initial part of the renal tubule is the highly twisted  proximal convoluted tubule (or proximal tubule), located in the cortex.  This is followed by the loop of Henle, a straight segment that dives deep into the medulla, makes a hairpin turn, and then ascends back toward the cortex.  The next segment, also in the cortex, is the smaller distal convoluted tubule (or distal tubule).  Connecting tubules (not labeled) link the distal tubule to the collecting duct, a structure that is found in both the cortex and the medulla.

The nephrons use three basic processes in the homeostatic regulation of the extracellular fluid, with the result being the formation of urine that drains from the collecting ducts.  These processes are depicted in the schematic nephron below, which has been untwisted and simplified to emphasize these processes.

schematic nephron with arrows indicating processes involved in formation of urine: glomerular filtration (green), reabsorption (cyan), and secretion (purple)
Schematic nephron showing the processes that occur in the formation of urine. The green arrow depicts glomerular filtration, which involves the bulk flow of plasma and solutes into Bowman’s space. The cyan arrows depict reabsorption (here indicated as tubular reabsorption), where substances are moved from the forming urine inside the renal tubule to the extracellular fluid. The purple arrows depict secretion (here indicated as tubular secretion), where substances are moved from the extracellular fluid into the forming urine.

First, blood plasma is filtered through a nonspecific process of bulk flow known as glomerular filtration.  This occurs in the renal corpuscle when the fluid and small molecules dissolved in plasma move from the inside of the glomerular capillaries into Bowman’s space, which is contained within Bowman’s capsule.

Next, the forming urine (or filtrate) flows through the renal tubule.  In the renal tubule, water and substances that the body needs to keep will be transported out of the lumen of the tubule and back into the extracellular fluid; this process is called reabsorption.  Certain substances that need to be eliminated from the body are transported from the extracellular fluid into the lumen of the tubule; this process is called secretion.  Both reabsorption and secretion depend on epithelial transport.  The movement of water depends upon osmosis.

 

Structures in the Renal Corpuscle

The renal corpuscles are where filtration occurs.  Renal corpuscles are found exclusively in the cortex.  The figure below shows a low-magnification view of the kidney cortex.

histology section showing the renal cortex at low power; the capsule is at the upper edge and renal corpuscles (green arrows) are scattered within the tissue
A section of kidney cortex. The capsule is indicated by the pink arrow. Some of the renal corpuscles are indicated by the bright green arrows.

The renal corpuscles are the blobs that are enclosed within the circular spaces.  A further indication that this section is from the cortex (besides the presence of corpuscles) is the connective tissue capsule seen at the top edge of the tissue section.

A higher magnification view of a renal corpuscle shows the glomerulus in the center, surrounded by Bowman’s capsule.

high magnification tissue section of a renal corpuscle; glomerulus indicated by bright green asterisk and outer edge of Bowman's capsule indicated by a black arrow
A higher magnification view showing a renal corpuscle. The glomerulus is indicated by the bright green asterisk. The black arrow indicates the simple squamous epithelium that forms the outside of Bowman’s capsule.

The outside of Bowman’s capsule, which encloses Bowman’s space, is formed by a simple squamous epithelium.  In the tissue surrounding the renal corpuscle, the circles and ovals formed by simple cuboidal epithelium are sections through the renal tubule, mainly sections of proximal tubules and distal tubules (see later in the chapter).

Structures Forming the Filtration Membrane

The figure below focusses in on the part of the schematic nephron depicting the renal corpuscle.

schematic drawing of renal corpuscle showing the outer and the inner surfaces of Bowman's capsule
Schematic drawing of a renal corpuscle showing the glomerulus (thin red line) surrounded by the outer and inner surfaces of Bowman’s capsule (blue).

In this figure, the glomerulus is simplified so that it appears as a single capillary loop.  Note that Bowman’s capsule consists of two surfaces.  One surface is the simple squamous epithelium that we just saw in the previous figure.  This surface forms the outside of Bowman’s capsule and encloses a space called Bowman’s space.  The other surface of Bowman’s capsule is the inner surface (also called the visceral surface) that surrounds the glomerular capillaries.  The inner surface of Bowman’s capsule is made up of a simple epithelium of podocytes, cells that wrap around the glomerular capillaries and form a part of the filtration membrane.

In the figure below, the podocytes are the pale lavender cells in the illustration of a renal corpuscle on the left.  The podocytes completely cover the endothelial cells making up the glomerular capillaries.

illustration of podocytes in a renal corpuscle (left) and a scanning EM of podocytes (right)
Left: a schematic drawing of the renal corpuscle, illustrating podocytes (light purple cells surrounding the glomerular capillaries). Right: Scanning electron micrograph showing podocytes wrapped around a capillary. Adapted from figures 19.5a and b in Human Physiology: An Integrated Approach by Dee Silverthorn, 8th edition.

The figure on the right is a scanning electron micrograph of the outer surface of a glomerular capillary, showing podocytes.  Podocytes have processes called arms that extend from their cell bodies.  Along the arms are smaller processes called foot processes that interdigitate with other foot processes.  (“Podo-” “-cyte” literally translates to “foot cell”.)

The endothelial cells that make up the glomerulus are specialized to favor filtration.  The glomerular capillary endothelial cells have fenestrations, which are pores that pas entirely through the cell.  The figure below illustrates the structure of a fenestrated capillary.

fenestrated capillary in whole view (top) and section (bottom)
Illustration showing a fenestrated capillary in whole view (top) and section (bottom). Figure 15.16b in Human Physiology: An Integrated Approach by Dee Silverthorn, 8th edition.

The figure below shows another scanning electron micrograph of a glomerulus.  This micrograph was prepared using the technique of freeze-fracture replication, in which frozen tissue is fractured, coated with heavy metals, and imaged using scanning electron microscopy.  The advantage of this technique is that it allows different surfaces of the tissue to be visualized.

scanning EM of glomerulus pseudo colored to show inside of fenestrated capillary in green and podocytes outside of capillary in yellow and blue
A pseudo-colored freeze-fracture scanning electron micrograph of a renal glomerulus. The inner surface of a glomerular capillary is colored green. The region enclosed in the yellow box shows podocytes (one colored blue, one colored yellow) wrapping around the outside of a capillary. The region inside the green and yellow boxes are enlarged in the next two figures. Image EM-234 from Histology Guide: Virtual Histology Lab https://www.histologyguide.com/EM-view/EM-234-kidney-glomerulus/16-photo-1.html

In this figure, the fracturing allows us to see both the inside and the outside of the glomerular capillaries.  This figure is pseudo-colored to help distinguish the inside of the capillary (green) from the outside covered by two podocytes (blue and yellow).  The green and yellow boxes indicate the regions that are enlarged in the next figures.

This figure (not pseudo-colored) shows the enlarged inner surface of the capillary.  The many small circles visible on the inner surface of the endothelial cell are fenestrations.

inner surface of a glomerular capillary showing fenestrations in an endothelial cell
Detail from freeze-fracture scanning EM showing the inside of a capillary loop. The endothelial cell (large cyan arrow) is filled with fenestrations (darker spots, some indicated by small yellow arrows).

This figure shows the enlarged outer surface of the capillary, showing in detail a podocyte, its arms, and its foot processes.

podocytes on the outer surface of a glomerular capillary loop; yellow arrow indicates larger arm while smaller cyan and green arrows indicate foot processes
Detail from freeze-fracture scanning EM showing the outside of a capillary loop, wrapped by podocytes. The podocyte has arms (yellow arrow) extending from its cell body, with foot processes (green and cyan arrows) extending from the arms.

The next figure shows how these two epithelia (the fenestrated endothelium of the capillary and the podocyte layer) combine to form the filtration membrane.

illustrations of a section through two capillary loops (left) and an enlargement (right) of the filtration membrane
Illustration showing cross section of two capillary loops in the glomerulus (left). On the right, the filtration membrane is enlarged. Figure 19.5c, d in Human Anatomy: An Integrated Approach by Dee Silverthorn, 8th edition.

On the left is a cross section showing two capillary loops within the glomerulus.  On the right is a highly magnified view of the filtration membrane.  The endothelium and the epithelium created by the podocytes have between them a shared basement membrane called the glomerular basement membrane.  The path of a filtered substance is first through a fenestration, then through the glomerular basement membrane, and finally through a filtration slit, which is the space between the podocyte foot processes.  A complex of proteins called the slit diaphragm spans the filtration slit.

Structures in the Renal Tubule

After moving into Bowman’s space, the forming urine (or filtrate) flows into the renal tubule.  The renal tubule is a hollow structure whose walls are formed by a simple epithelium.  Through most of the renal tubule, this is a simple cuboidal epithelium, the exception being part of the loops of Henle.  Different regions of the renal tubule express specific transport proteins and are key for certain processes.  The functions of each part of the nephron are summarized in the figure below.

schematic nephron showing key functions in different regions
Key functions in different parts of the nephron. Adapted from figure 19.2 in Human Anatomy: An Integrated Approach by Dee Silverthorn, 8th edition.

We will examine the histology of two parts of the renal tubule:  the proximal tubule and the collecting duct.

Proximal Tubule

The figure below shows a section through the renal cortex, which contains renal corpuscles as well as proximal and distal tubules and the first part of the collecting duct.  This figureacaptures Bowman’s space opening up into a proximal tubule.

light micrograph of renal cortex showing two renal corpuscles and sections through the renal tubule; proximal tubules are intensely stained and indicated with an arrow (attached to Bowman's space) and asterisks
Light micrograph showing renal cortex. The proximal tubule indicated with an arrow can be identified as such because it is connected to Bowman’s space. Interiors of other proximal tubules are marked with asterisks.
light micrograph with renal corpuscle and surrounding renal tubules; asterisks mark intensely stained proximal tubules
Light micrograph of renal cortex. Interiors of proximal tubules marked with asterisks.

The proximal tubule is the major site for reabsorption and secretion in the renal tubule.  Because these are energy-dependent processes that utilize ATP, the proximal tubule is full of mitochondria.  In these two light micrographs, the mitochondria stain with the bright pink dye, and so the proximal tubules are the circles and ovals whose epithelium is more intensely stained.  The cuboidal epithelium in the proximal tubule also has a fuzzy appearance due to the fact that the cell membranes are highly folded.  The lighter stained tubules are distal tubules and potentially a few cortical collecting ducts.

The next figure is a transmission electron micrograph of the proximal tubule epithelium.

wheater

The electron micrograph shows that proximal tubule cells are filled with mitochondria, which appear as grey ovals in the cytoplasm of the cells.  The apical membrane (at the top) is folded into microvilli, which increases the amount of surface area available for the expression of membrane transport proteins.  The basolateral membrane (bottom) is also folded into larger membrane processes.

Collecting Duct

The collecting duct is involved in regulated reabsorption of sodium and water and is also involved in acid-base balance.  We will examine the medullary collecting duct, which plays a key role in the regulated absorption of water.

To look at the histology of the collecting duct, we will take advantage of the structure of the rodent kidney, which, unlike a human kidney, has a single papilla.  This means that a cross-section of the tissue will be organized into distinct regions of cortex, outer medulla (closest to the cortex), and inner medulla (closest to the papilla).

Longitudinal (left) and transverse (right) sections of unipapillar rodent kidney. Green line in right figure illustrates plane of section for left hand figure, which shows the cortex, medulla, papilla, and calyx
Left: longitudinal section of a unipapillar rodent kidney. The green line indicates the plane of section for the figure at right (which utilizes a different staining technique). The transverse section on the right indicates the arrangement of cortex and medulla, as well as the location of the papilla and calyx. Source for lefthand figure: https://ntp.niehs.nih.gov/nnl/urinary/kidney/index.htm

The inner part of the medulla contains only collecting ducts and thin segments of the loop of Henle.  The thin segments of the loop of Henle are made up of simple squamous epithelium, while the epithelium of the collecting duct is simple cuboidal epithelium.

tissue section from the inner medulla showing collecting ducts indicated by red arrows
A section through the inner region of the medulla. Collecting ducts (interiors indicated by small red arrows) are oriented vertically in the figure. Red blood cells visible in the upper half of the right-most collecting duct are an artefact of tissue processing.

The high powered micrograph above shows several collecting ducts.  A characteristic of the collecting duct is the crisp, clear outlines of the cells in the cuboidal epithelium.

Histology of the Collecting System and Bladder

The collecting system of the kidney and the bladder are lined with a special type of stratified epithelium called uroepithelium or transitional epithelium. (Recall that a stratified epithelium is one that is made up of multiple layers of cells).

drawing of uroepithelium
Illustration of the uroepithelium with the basement membrane (BM) at the bottom and umbrella cells (U) at the apical surface (top). Figure 5.8a in Wheater’s Functional Histology, 7th edition.

The uroepithelium possesses two key features:

  1. It can adapt to the changing volume of the bladder and collecting structures.  The uroepithelium stretches when these structures are full of urine and distended, and it folds up like an accordion when these structures have a small volume.  There are specialized cells called umbrella cells (labeled with a U in the figure above) that are located at the apical surface of the uroepithelium that can change their size and shape as structures fill with urine.
  2. It is largely impermeant to water and toxins.  Thus concentrated or dilute urine maintains its state while it is being stored in the bladder.  Umbrella cells are important in the barrier function of the uroepithelium.

The figure below illustrates the transitional nature of the uroepithelium in the bladder, comparing its appearance in a relaxed and distended state.

section showing uroepithelium (at top) in relaxed (left) and distended (right) bladder
Uroepithelium in relaxed (left) and distended (right) bladder. Adapted from image MH018 from Histology Guide: Virtual Histology Lab. https://histologyguide.com/slideview/MH-018-transitional-epithelia/16-slide-1.html

The first example of uroepithelium that we will examine is from a calyx in a rodent kidney.  At low magnification, one can see the papilla, containing sectioned collecting ducts, as it fits into the calyx.

papilla (top) inside a calyx
The papilla and calyx from a rodent kidney. Collecting ducts are indicated by red arrows. The calyx is lined with uroepithelium.

The calyx is lined with uroepithelium, which is shown at high magnification in the figure below.

uroepithelium on surface of calyx at high magnification
High magnification view of uroepithelium on a calyx.

The next figure shows a low magnification view of a section through the entire bladder.

section of bladder at low power indicating region enlarged in subsequent figures
Low power view of a section of the bladder. The blue box outlines the region that is enlarged in the figure showing the detrusor muscle. The small black box outlines the region that is enlarged to show the uroepithelium. Image MHS 214 from Histology Guide: Virtual Histology Lab
https://histologyguide.com/slideview/MHS-214-bladder/16-slide-1.html

The bladder wall is mostly made up of irregularly arranged smooth muscle which is known as the detrusor muscle.

 

outer wall of bladder wooing detrusor muscle indicated by green bracket
High magnification of the outer wall of the bladder showing the detrusor muscle. The detrusor muscle (outlined by green bracket) consists of bundles of smooth muscle cells that are oriented in many different directions. Inset at upper left shows the low power view of the whole bladder.

The bladder wall also contains some connective tissue.  The inner surface of the bladder is lined with uroepithelium.

inner surface of bladder wall showing uroepithelium indicated by green bracket
High magnification of the inner wall of the bladder showing the uroepithelium, indicated by the green bracket.

 

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375-Human Physiology in Health and Disease (PBIO 375) Copyright © by Anna Melby. All Rights Reserved.